Browsing by Author "Lin, Chin-Hsien"

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    Development of an ultra-high sensitive immunoassay with plasma biomarker for differentiating Parkinson disease dementia from Parkinson disease using antibody functionalized magnetic nanoparticles
    (2016-06-08) Yang, Shieh-Yueh; Chiu, Ming-Jang; Lin, Chin-Hsien; Horng, Herng-Er; Yang, Che-Chuan; Chieh, Jen-Jie; Chen, Hsin-Hsien; Liu, Bing-Hsien
    Abstract Background It is difficult to discriminate healthy subjects and patients with Parkinson disease (PD) or Parkinson disease dementia (PDD) by assaying plasma α-synuclein because the concentrations of circulating α-synuclein in the blood are almost the same as the low-detection limit using current immunoassays, such as enzyme-linked immunosorbent assay. In this work, an ultra-sensitive immunoassay utilizing immunomagnetic reduction (IMR) is developed. The reagent for IMR consists of magnetic nanoparticles functionalized with antibodies against α-synuclein and dispersed in pH-7.2 phosphate-buffered saline. A high-Tc superconducting-quantum-interference-device (SQUID) alternative-current magnetosusceptometer is used to measure the IMR signal of the reagent due to the association between magnetic nanoparticles and α-synuclein molecules. Results According to the experimental α-synuclein concentration dependent IMR signal, the low-detection limit is 0.3 fg/ml and the dynamic range is 310 pg/ml. The preliminary results show the plasma α-synuclein for PD patients distributes from 6 to 30 fg/ml. For PDD patients, the concentration of plasma α-synuclein varies from 0.1 to 100 pg/ml. Whereas the concentration of plasma α-synuclein for healthy subjects is significantly lower than that of PD patients. Conclusions The ultra-sensitive IMR by utilizing antibody-functionalized magnetic nanoparticles and high-Tc SQUID magnetometer is promising as a method to assay plasma α-synuclein, which is a potential biomarker for discriminating patients with PD or PDD.
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    高職生國文自傳寫作病句辨識與網路評閱機制對寫作成效的影響研究
    (2015) 林金賢; Lin, Chin-Hsien
    本研究針對國文自傳寫作中的病句辨識部分，結合配對式合作學習，並且應用網路評閱機制，探詢是否能夠提升學習者的病句辨識成效和自傳寫作成效。本研究在桃園區某國立綜合高中進行準實驗，實驗分成實驗組A、B和對照組，共有92受試者，三組接受病句辨識教學，皆使用網路平台進行評閱：實驗組A為使用級分和評語的同儕互評；實驗組B是僅使用級分的同儕互評；對照組則是使用級分和評語的教師評閱，探求不同的網路評閱方式之下，受試者的病句辨識能力學習成效、寫作成效的影響。本研究結果顯示級分和評語的同儕互評的實驗組A，其病句辨識有顯著成效，且在寫作成效後測中優於另外兩組。 根據受試者不同評閱階段，蒐集病句語料進行出現頻率分析，在國文自傳寫作評閱三階段中，標點錯漏、未用全形、錯誤字形為出現頻率最高的病句種類，也為高職自傳寫作中首要的教學點；而隨著對寫作主題的確認，反而多餘成分的病句類別會再次一階段出現，待下一階段修訂作品而減少。病句辨識或評閱活動宜分階段主題進行。