Browsing by Author "Wu, Wei-Ren"

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    Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay
    (2018-05-16) Chiu, Nan-Fu; Chen, Chi-Chu; Yang, Cheng-Du; Kao, Yu-Sheng; Wu, Wei-Ren
    Abstract In this study, we propose a modified gold nanoparticle-graphene oxide sheet (AuNP-GO) nanocomposite to detect two different interactions between proteins and hybrid nanocomposites for use in biomedical applications. GO sheets have high bioaffinity, which facilitates the attachment of biomolecules to carboxyl groups and has led to its use in the development of sensing mechanisms. When GO sheets are decorated with AuNPs, they introduce localized surface plasmon resonance (LSPR) in the resonance energy transfer of spectral changes. Our results suggest a promising future for AuNP-GO-based label-free immunoassays to detect disease biomarkers and rapidly diagnose infectious diseases. The results showed the detection of antiBSA in 10 ng/ml of hCG non-specific interfering protein with dynamic responses ranging from 1.45 nM to 145 fM, and a LOD of 145 fM. Considering the wide range of potential applications of GO sheets as a host material for a variety of nanoparticles, the approach developed here may be beneficial for the future integration of nanoparticles with GO nanosheets for blood sensing. The excellent anti-interference characteristics allow for the use of the biosensor in clinical analysis and point-of-care testing (POCT) diagnostics of rapid immunoassay products, and it may also be a potential tool for the measurement of biomarkers in human serum.
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    以石墨烯量子點調制表面電漿子能量於生物感測器之研究
    (2018) 吳瑋仁; Wu, Wei-Ren
    疾病的早期發現早期治療，是近代醫學的檢測與診斷的方向，生物醫學晶片能夠提供快速、準確、低成本的檢測，以實現精準醫療的目標。本研究提出利用石墨烯量子點(GQDs)結合表面電漿子共振(SPR)技術來開發能夠快速檢測胰臟癌疾病的生物晶片。 石墨烯量子點是二維的新穎材料，具有良好的生物相容性與優異的光致發光之螢光效益。因此本研究使用水熱合成法自行合成石墨烯量子點，並進行光學特性分析、元素分析以及形態分析驗的驗證，證實透過材料顆粒大小可以調控光致發光的螢光顏色。在石墨烯量子點修飾於金奈米粒子的實驗中，發現石墨烯量子點和金奈米粒子之間的交互作用，能誘導表面電漿子的能量轉移特性產生耦合現象，並藉由調制表面電漿子能量來控制螢光的色變，開發螢光生物感測技術。 石墨烯量子點藉由表面電漿子共振技術檢測生物分子間的交互作用，進行檢測胰臟癌腫瘤指標物CA19-9之免疫反應的實驗，透過快速低成本的檢測技術進而開發高靈敏度及高準確性之快速篩檢的生物感測器。本實驗最低檢測可達約10 Unit/ml的變化，且線性迴歸係數可達R2 = 0.9，透過此檢測技術即能快速診斷胰臟癌。由此實驗可證明石墨烯量子點具有良好的生物相容性，結合生物晶片亦能提高檢測的靈敏度，並能夠用於開發具有專一性的快速篩檢的癌症生物感測晶片。