瀕絕物種烏來杜鵑的族群內遺傳變異研究
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Date
1995-10-??
Authors
黃生
陳兆美
許素玲
呂勝由
S.Huang,C.M.Chen,S.LHsu,S.Y.Lu
Journal Title
Journal ISSN
Volume Title
Publisher
國立臺灣師範大學生命科學學系
Department of Life Science, NTNU
Department of Life Science, NTNU
Abstract
烏來杜鵑(Rhododendron kanehirai Wilson)天然族群己因原生育地破壞而消失，區外保育(ex situ conservation)為本種復育工作的第一步。研究其遺傳變異皮之高低，復為更迫切之工作。本報告採取保存於臺灣省林業試驗所苗圃中收集的烏來杜鵑60個單株為樣品，逐個進行同功酶檢測，結果發現在15種酵素中，多型對偶基因僅存於PER,SKDH, PGI-2 , LAP-2等4個基因座上。多型基因百分比為16.7%。其中除PER普通呈多型性存於族群內之外，其餘各式多型基因分別分佈在10株個體中。依照此初步數據判斷，本種的復育在方法上，應先行繁殖這10株保有基因多型性的殘存個體，以維持其族群比例(population ratio)。並且在增加數量的過程中能保持足夠的有效族群大小(effective population size (Ne))，以免在復育過程中，因選擇不當，盲目繁殖大量同質個體造成基因僵化( fixed)，反致加速該物種之滅絕。
Rhododendron kanehirai Wilson is an endemic species of Taiwan. It occurs only on steep hanks along the Pei-Shih creek in restricted areas of northern Taiwan. R. kallehirai has heen extincted due to the hahitat destruction –the construction of the Emerald Reservoir. fly 1992, the number of individuals of this species reduced helow 100 and remained only in a nursery in Taiwan Forestry Research Institute in Taipei. Artificial by means of cuttings trom these survivors is primarily, and in Taiwan exclusively way for propagation. However, for restoration of this species, to investigate the genetic variation within small popUlation will be important. In this report, starch gelelectrophoresis was used to study the isozyme phenotypes of 60 individuals of R. kanehirai. Four of the eighteen sllrveyed genetic markers (SKDH, PGI, PER and LAP) represent polymorphisms. Genetie variation is low of this species (He= 0.(64). Mean number and effective number of alleles per locus (A=1.20; Ae=UJ7) are also low. Allozyme data of this report indicates that restricted gene flow and genetic drill in small and isolated population may reduce genetic variahility These results are discussed in tenus of the strategies for ex situ conservation and population restoration.
Rhododendron kanehirai Wilson is an endemic species of Taiwan. It occurs only on steep hanks along the Pei-Shih creek in restricted areas of northern Taiwan. R. kallehirai has heen extincted due to the hahitat destruction –the construction of the Emerald Reservoir. fly 1992, the number of individuals of this species reduced helow 100 and remained only in a nursery in Taiwan Forestry Research Institute in Taipei. Artificial by means of cuttings trom these survivors is primarily, and in Taiwan exclusively way for propagation. However, for restoration of this species, to investigate the genetic variation within small popUlation will be important. In this report, starch gelelectrophoresis was used to study the isozyme phenotypes of 60 individuals of R. kanehirai. Four of the eighteen sllrveyed genetic markers (SKDH, PGI, PER and LAP) represent polymorphisms. Genetie variation is low of this species (He= 0.(64). Mean number and effective number of alleles per locus (A=1.20; Ae=UJ7) are also low. Allozyme data of this report indicates that restricted gene flow and genetic drill in small and isolated population may reduce genetic variahility These results are discussed in tenus of the strategies for ex situ conservation and population restoration.