A Study on Integrated Dual-Mode Holographic Tomography and Adaptive Wavefront Correction Technique for Free- Floating Single Live Cell Label-Free Imaging

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dc.contributor鄭超仁zh_TW
dc.contributorCheng, Chau-Jernen_US
dc.contributor.authorB. Vinothzh_TW
dc.contributor.authorBALASUBRAMANI VINOTHen_US
dc.date.accessioned2020-10-19T07:12:26Z
dc.date.available不公開
dc.date.available2020-10-19T07:12:26Z
dc.date.issued2019
dc.description.abstractnonezh_TW
dc.description.abstractThree-dimensional (3D) label-free refractive index (RI) imaging and analysis of a free-floating single live cell is a puzzling keyword for the biological research communities. Digital holographic tomography (DHT) is a potential technique to generate 3D RI profile of the biological specimens without labelling. The DHT uses interferometer configuration followed by either by sample rotation method or by beam rotation method. However, there is no such experimental system is developed to perform both full-angle sample rotation method and beam rotation method; this study developed such an integrated dual-mode tomography (IDT) system for the first time. The IDT system is developed by combining holographic optical tweezers (HOT) with digital holographic microscopy (DHM). The HOT system is used to control the free-floating live sample and the DHM records the transmitted wavefronts of the sample simultaneously. In this manner, the developed IDT system is capable of collecting and combing the spatial frequencies of full angle sample rotation with beam rotation method to extend the spatial frequency coverages along lateral and axial directions. Consequently, a novel unidentified flying object (UFO) like shaped experimental transfer function is obtained. To demonstrate the potential capability of the developed IDT method, a free-floating live Candida rugosa were used as a sample and its label-free 3D RI profile is generated at its sub-cellular level. The experimental results revealed that the IDT method can quantitatively enhance the lateral and axial resolutions without using any complicated image processing algorithm One of the major issues in an imaging system is the aberrations induced by the optical components and its alignments, which severely degrades the resolution and the imaging performance of the experimental system. This research study also focused on the development of a novel computer-generated hologram (CGH) based adaptive wavefront compensation technique demonstrated on a compactly developed structured illumination holographic tomography (SI-HT) system. A series of CGHs comprises of phase Fresnel lens and binary blazed grating are designed and displayed on a phase-only spatial light modulator to generate structured light pattern on the sample. The method is validated using Siemens star target and the potential application is verified using live sample candiada rugosa and its sub-cellular level 3D label-free RI profile is generated. The experimental results demonstrated the capability of the method to enhance the resolution in lateral and axial directions.en_US
dc.description.sponsorship光電工程研究所zh_TW
dc.identifierG080448002S
dc.identifier.urihttp://etds.lib.ntnu.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=id=%22G080448002S%22.&%22.id.&
dc.identifier.urihttp://rportal.lib.ntnu.edu.tw:80/handle/20.500.12235/112050
dc.language英文
dc.subjectdigital holographyzh_TW
dc.subjectmicroscopyzh_TW
dc.subjectthree-dimensional imagingzh_TW
dc.subjectbeam rotation tomographyzh_TW
dc.subjectsample rotation tomographyzh_TW
dc.subjectsingle cell analysiszh_TW
dc.subjectlabel-free imagingzh_TW
dc.subjectaberration correctionzh_TW
dc.subjectadaptive opticszh_TW
dc.subjectstructured illuminationzh_TW
dc.subjectdigital holographyen_US
dc.subjectmicroscopyen_US
dc.subjectthree-dimensional imagingen_US
dc.subjectbeam rotation tomographyen_US
dc.subjectsample rotation tomographyen_US
dc.subjectsingle cell analysisen_US
dc.subjectlabel-free imagingen_US
dc.subjectaberration correctionen_US
dc.subjectadaptive opticsen_US
dc.subjectstructured illuminationen_US
dc.titleA Study on Integrated Dual-Mode Holographic Tomography and Adaptive Wavefront Correction Technique for Free- Floating Single Live Cell Label-Free Imagingzh_TW
dc.titleA Study on Integrated Dual-Mode Holographic Tomography and Adaptive Wavefront Correction Technique for Free- Floating Single Live Cell Label-Free Imagingen_US

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